DB43/T2678—2023 43 湖南省地方标准 ICS CCS 65.020.01 B 39 发 布 湖南省市场监督管理局蛹虫草菌种生产技术规程 Technology Code of practice of production for yongchongcao strain production 2023-0 8发布-22 2023-11实施-2 2 DB43/T 2678—2023 I 目 次 前言 ································ ································ ································ ························ Ⅲ 1 范围 ································ ································ ································ ····················· 1 2 规范性引用文件 ································ ································ ································ ······ 1 3 术语和定义 ································ ································ ································ ············ 1 4 环境条件 ································ ································ ································ ··············· 1 5 菌种选择 ································ ································ ································ ··············· 1 6 母种生产 ································ ································ ································ ··············· 2 6.1 工艺流程 ································ ································ ································ ········· 2 6.2 培养基配制 ································ ································ ································ ······ 2 6.3 分装与灭菌 ································ ································ ································ ······ 2 6.4 斜面摆放 ································ ································ ································ ········· 2 6.5 接种 ································ ································ ································ ··············· 2 6.6 培养 ································ ································ ································ ··············· 2 6.7 贮存 ································ ································ ································ ··············· 2 7 栽培种生产 ································ ································ ································ ············ 2 7.1 工艺流程 ································ ································ ································ ········· 2 7.2 培养基配制 ································ ································ ································ ······ 2 7.3 分装与灭菌、冷却 ································ ································ ····························· 2 7.4 接种 ································ ································ ································ ··············· 3 7.5 培养 ································ ································ ································ ··············· 3 7.6 贮存 ································ ································ ································ ··············· 3 8 质量检验 ································ ································ ································ ··············· 3 8.1 质量要求 ································ ································ ································ ········· 3 8.2 抽样与检测 ································ ································ ································ ······ 3 8.3 留样 ································ ································ ································ ··············· 3 8.4 标识与储运 ································ ································ ································ ······ 3 9 废弃物处理 ································ ································ ································ ············ 3 10 档案管理 ································ ································ ································ ·············· 3 附录A（资料性） 常用母种培养基配方 ································ ································ ·········· 4 附录B（资料性） 常用栽培种培养基配方 ································ ································ ······· 5 附录C（资料性） 母种质量要求 ································ ·